Difference between revisions of "Part:BBa K4759025:Design"
JianghuaChen (Talk | contribs) |
|||
(One intermediate revision by one other user not shown) | |||
Line 6: | Line 6: | ||
− | ===Design | + | ===Design=== |
Recombinant plasmid pCDFDuet-hemA-hemL is expressed in engineered bacteria | Recombinant plasmid pCDFDuet-hemA-hemL is expressed in engineered bacteria | ||
Line 16: | Line 16: | ||
===References=== | ===References=== | ||
+ | |||
+ | [1]Sultana, N. Microbial biotransformation of bioactive and clinically useful steroids and some salient features of steroids and biotransformation. Steroids 2018, 136. DOI: 10.1016/j.steroids.2018.01.007. |
Latest revision as of 17:07, 11 October 2023
hemL
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1028
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1028
Illegal NheI site found at 738 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1028
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1028
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1028
Illegal AgeI site found at 388
Illegal AgeI site found at 721 - 1000COMPATIBLE WITH RFC[1000]
Design
Recombinant plasmid pCDFDuet-hemA-hemL is expressed in engineered bacteria
Source
A genecoding for glutamate-1-semialdehyde 2,1-aminomutase (GSAM).
References
[1]Sultana, N. Microbial biotransformation of bioactive and clinically useful steroids and some salient features of steroids and biotransformation. Steroids 2018, 136. DOI: 10.1016/j.steroids.2018.01.007.