Difference between revisions of "Part:BBa K4759021:Design"
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− | ===Design | + | ===Design=== |
Recombinant plasmid pETDuet-hemB-hemC-hemD-hemH is expressed in engineered bacteria | Recombinant plasmid pETDuet-hemB-hemC-hemD-hemH is expressed in engineered bacteria | ||
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===References=== | ===References=== | ||
+ | [1] Sultana, N. Microbial biotransformation of bioactive and clinically useful steroids and some salient features of steroids and biotransformation. Steroids 2018, 136. DOI: 10.1016/j.steroids.2018.01.007. | ||
+ | [2] Watchmaker, J.; Callaghan, D. J., III; Dover, J. S. Deoxycholic Acid in Aesthetic Medicine. Advances in Cosmetic Surgery 2020, 3 (1), 77-87. DOI: 10.1016/j.yacs.2020.01.009 |
Latest revision as of 17:14, 11 October 2023
hemB
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 467
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 467
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 467
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 467
- 1000COMPATIBLE WITH RFC[1000]
Design
Recombinant plasmid pETDuet-hemB-hemC-hemD-hemH is expressed in engineered bacteria
Source
A gene coding for porphobilinogen synthase (PBGS),The enzyme catalyzes the asymmetric condensation and cyclization of two 5-aminolevulinate molecules
References
[1] Sultana, N. Microbial biotransformation of bioactive and clinically useful steroids and some salient features of steroids and biotransformation. Steroids 2018, 136. DOI: 10.1016/j.steroids.2018.01.007. [2] Watchmaker, J.; Callaghan, D. J., III; Dover, J. S. Deoxycholic Acid in Aesthetic Medicine. Advances in Cosmetic Surgery 2020, 3 (1), 77-87. DOI: 10.1016/j.yacs.2020.01.009