Difference between revisions of "Part:BBa K4765102"

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===Usage and Biology===
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Similar to [https://parts.igem.org/Part:BBa_K4765104 BBa_K4765104(ribozyme+strong RBS+intimin-Nb2 fusion+stem-loop)], we replaced Nb2 with Nb1 in order to interact with [https://parts.igem.org/Part:BBa_K4765101 BBa_K4765101(ribozyme+strong RBS+intimin-Ag1 fusion+stem-loop)] to create a biofilm.
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Similar to [https://parts.igem.org/Part:BBa_K4765106 BBa_K4765106], we replaced Nb3 with Nb1 in order to interact with [https://parts.igem.org/Part:BBa_K4765101 BBa_K4765101] to create a biofilm.
  
 
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Latest revision as of 15:42, 12 October 2023


Twister P1 + T7_RBS + intimin-Nb1 fusion + stem-loop

contributed by Fudan iGEM 2023


Similar to BBa_K4765106, we replaced Nb3 with Nb1 in order to interact with BBa_K4765101 to create a biofilm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2075
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1305