Difference between revisions of "Part:BBa K4765114"

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===Introduction===
 
===Introduction===
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This composite part is composed of Dsup and ribozyme-assisted polycistronic co-expression system：pRAP. Dsup is a protein that helps protect DNA from radiation damage. It was discovered in the Tardigrade Species ''Ramazzottius Varieornatus'' and tested by iGEM17_WashU_StLouis in [https://parts.igem.org/Part:BBa_K2195000  Dsup (Radiation Protection)]
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This composite part is composed of Rv Dsup and ribozyme-assisted polycistronic co-expression system：pRAP. Dsup is a nucleosome-binding protein that helps protect DNA from radiation damage. It was discovered in the Tardigrade Species ''Ramazzottius Varieornatus'' .
  
 
===Usage and Biology===
 
===Usage and Biology===
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We constructed Dsup into pRAP to endow ''E. coli'' with anti-UV capability.
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We constructed Rv Dsup into pRAP to endow ''E. coli'' with anti-UV capability.
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===Characterization===
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4765114 SequenceAndFeatures</partinfo>
 
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<partinfo>BBa_K4765114 parameters</partinfo>
 
<partinfo>BBa_K4765114 parameters</partinfo>
 
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==Reference==
 

Latest revision as of 15:51, 12 October 2023


Twister P1 + T7_RBS + Rv Dsup + stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of Rv Dsup and ribozyme-assisted polycistronic co-expression system：pRAP. Dsup is a nucleosome-binding protein that helps protect DNA from radiation damage. It was discovered in the Tardigrade Species Ramazzottius Varieornatus .

Usage and Biology

We constructed Rv Dsup into pRAP to endow E. coli with anti-UV capability.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 230
    Illegal AgeI site found at 301
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 227
    Illegal SapI.rc site found at 956