Difference between revisions of "Part:BBa K4765020"
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===Introduction=== | ===Introduction=== | ||
| − | The twister ribozyme is an RNA molecule that is capable of self-cleavage at specific sites.The RNA exhibits nucleolytic ribozyme activity both in vitro and in vivo, with cleavage rates comparable to those of other nucleolytic ribozymes<ref>Eiler, D., Wang, J., & Steitz, T. A. (2014). Structural basis for the fast self-cleavage reaction catalyzed by the twister ribozyme. ''Proceedings of the National Academy of Sciences, 111''(36), 13028–13033. https://doi.org/10.1073/pnas.1414571111</ref>. This grants it the ability to regulate polycistronic co-expression system<ref> Liu, Y., Wu, Z., Wu, D., Gao, N., & Lin, J. (2022). Reconstitution of Multi-Protein Complexes through Ribozyme-Assisted Polycistronic Co-Expression. ''ACS Synthetic Biology, 12''(1), 136–143. https://doi.org/10.1021/acssynbio.2c00416</ref> | + | The twister ribozyme is an RNA molecule that is capable of self-cleavage at specific sites.The RNA exhibits nucleolytic ribozyme activity both ''in vitro'' and ''in vivo'', with cleavage rates comparable to those of other nucleolytic ribozymes<ref>Eiler, D., Wang, J., & Steitz, T. A. (2014). Structural basis for the fast self-cleavage reaction catalyzed by the twister ribozyme. ''Proceedings of the National Academy of Sciences, 111''(36), 13028–13033. https://doi.org/10.1073/pnas.1414571111</ref>. This grants it the ability to regulate polycistronic co-expression system<ref> Liu, Y., Wu, Z., Wu, D., Gao, N., & Lin, J. (2022). Reconstitution of Multi-Protein Complexes through Ribozyme-Assisted Polycistronic Co-Expression. ''ACS Synthetic Biology, 12''(1), 136–143. https://doi.org/10.1021/acssynbio.2c00416</ref> |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | The Twister ribozyme is a crucial component of | + | The Twister ribozyme is a crucial component of ribozyme-assisted polycistronic co-expression system. |
| − | We incorporate the twister ribozyme between the coding CDSs in a polycistron. This ribozyme cleaves the polycistronic mRNA transcript, converting them into individual mono-cistrons. This process eliminates interactions between the cistrons, ensuring each mono-cistron has a comparable translation initiation rate. Subsequently, in conjunction with the RBS designed by the | + | We incorporate the twister ribozyme between the coding CDSs in a polycistron. This ribozyme cleaves the polycistronic mRNA transcript, converting them into individual mono-cistrons. This process eliminates interactions between the cistrons, ensuring each mono-cistron has a comparable translation initiation rate. Subsequently, in conjunction with the RBS designed by the software RAP, we can modulate the translation initiation rate of individual mono-cistrons, enabling precise adjustment of the pRAP co-expression system. |
===Characterization=== | ===Characterization=== | ||
| + | Details in [https://parts.igem.org/Part:BBa_K4765120 BBa_K4765120] | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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<partinfo>BBa_K4765020 parameters</partinfo> | <partinfo>BBa_K4765020 parameters</partinfo> | ||
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==References== | ==References== | ||
<references /> | <references /> | ||
Latest revision as of 12:11, 12 October 2023
Twister P1 ribozyme, from 10.1080/15476286.2022.2123640
Introduction
The twister ribozyme is an RNA molecule that is capable of self-cleavage at specific sites.The RNA exhibits nucleolytic ribozyme activity both in vitro and in vivo, with cleavage rates comparable to those of other nucleolytic ribozymes[1]. This grants it the ability to regulate polycistronic co-expression system[2]
Usage and Biology
The Twister ribozyme is a crucial component of ribozyme-assisted polycistronic co-expression system.
We incorporate the twister ribozyme between the coding CDSs in a polycistron. This ribozyme cleaves the polycistronic mRNA transcript, converting them into individual mono-cistrons. This process eliminates interactions between the cistrons, ensuring each mono-cistron has a comparable translation initiation rate. Subsequently, in conjunction with the RBS designed by the software RAP, we can modulate the translation initiation rate of individual mono-cistrons, enabling precise adjustment of the pRAP co-expression system.
Characterization
Details in BBa_K4765120
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Eiler, D., Wang, J., & Steitz, T. A. (2014). Structural basis for the fast self-cleavage reaction catalyzed by the twister ribozyme. Proceedings of the National Academy of Sciences, 111(36), 13028–13033. https://doi.org/10.1073/pnas.1414571111
- ↑ Liu, Y., Wu, Z., Wu, D., Gao, N., & Lin, J. (2022). Reconstitution of Multi-Protein Complexes through Ribozyme-Assisted Polycistronic Co-Expression. ACS Synthetic Biology, 12(1), 136–143. https://doi.org/10.1021/acssynbio.2c00416