Difference between revisions of "Part:BBa K4765005"
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===Introduction=== | ===Introduction=== | ||
| − | Nb2 is a variant of nanobody, a type of single-domain antibody<ref>Glass, D. S., & Riedel-Kruse, I. H. (2018). A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. Cell, 174(3), 649-658.e16. https://doi.org/10.1016/j.cell.2018.06.041 | + | Nb2 is a variant of nanobody, a type of single-domain antibody<ref>Glass, D. S., & Riedel-Kruse, I. H. (2018). A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. ''Cell, 174''(3), 649-658.e16. https://doi.org/10.1016/j.cell.2018.06.041 |
</ref> | </ref> | ||
| − | . It can interact with [https://parts.igem.org/Part:BBa_K4765004 Ag2]and the interaction between Ag-Nb can mediate specific | + | . It can interact with [https://parts.igem.org/Part:BBa_K4765004 BBa_K4765004(Ag2)]and the interaction between Ag-Nb can mediate specific binding between ''E.coli''. |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | The combination of | + | The combination of Nb2's single-domain structure and the intimin surface display system allows the entirety of a highly specific, cell surface-bound adhesin to be encoded as a single fusion protein. |
===Characterization=== | ===Characterization=== | ||
Latest revision as of 15:14, 12 October 2023
Nb2, antiEPEA from 10.1016/j.cell.2018.06.041
Introduction
Nb2 is a variant of nanobody, a type of single-domain antibody[1] . It can interact with BBa_K4765004(Ag2)and the interaction between Ag-Nb can mediate specific binding between E.coli.
Usage and Biology
The combination of Nb2's single-domain structure and the intimin surface display system allows the entirety of a highly specific, cell surface-bound adhesin to be encoded as a single fusion protein.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
- ↑ Glass, D. S., & Riedel-Kruse, I. H. (2018). A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. Cell, 174(3), 649-658.e16. https://doi.org/10.1016/j.cell.2018.06.041