Difference between revisions of "Part:BBa K218001:Design"
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<partinfo>BBa_K218001 short</partinfo> | <partinfo>BBa_K218001 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | This part was submitted to the Registry in 2008 ( | + | This part was submitted to the Registry in 2008 ([[Part:BBa_K131016]]); however, this part was cloned again and sequenced and is being re-submitted. |
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+ | LuxO and LuxU are found within the operon, therefore we have decided to keep these genes together. The internal RBS was also kept. | ||
===Source=== | ===Source=== | ||
GenBank Accession# L26221 LuxO Protein ID# AAD12736.1 LuxU Protein ID# AAD12737.2 | GenBank Accession# L26221 LuxO Protein ID# AAD12736.1 LuxU Protein ID# AAD12737.2 | ||
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===References=== | ===References=== | ||
+ | Jeremy A. Freeman and Bonnie L. Bassler. Sequence and function of LuxU: a two-component phosphorelay protein that regulates quorum sensing in Vibrio harveyi. 1999. Journal of Bacteriology. Feb 1999, p. 899-906. |
Latest revision as of 02:53, 22 October 2009
LuxOU from Vibrio harveyi
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 854
Illegal SapI.rc site found at 816
Design Notes
This part was submitted to the Registry in 2008 (Part:BBa_K131016); however, this part was cloned again and sequenced and is being re-submitted.
LuxO and LuxU are found within the operon, therefore we have decided to keep these genes together. The internal RBS was also kept.
Source
GenBank Accession# L26221 LuxO Protein ID# AAD12736.1 LuxU Protein ID# AAD12737.2
References
Jeremy A. Freeman and Bonnie L. Bassler. Sequence and function of LuxU: a two-component phosphorelay protein that regulates quorum sensing in Vibrio harveyi. 1999. Journal of Bacteriology. Feb 1999, p. 899-906.