Difference between revisions of "Part:BBa K260002"
 
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The pSC101 origin of replication is temperature sensitive due to a mutation of repA. Permisive temperature is 30 degC. 37 degC should be avoided as the plasmid can not replicate.
 
The pSC101 origin of replication is temperature sensitive due to a mutation of repA. Permisive temperature is 30 degC. 37 degC should be avoided as the plasmid can not replicate.
  
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This plasmid backbone is available with part BBa_K260017, with which it forms a functional unit: [anhydrotetracycline] -> [FLP] -> RFP
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This plasmid backbone is available with part [[Part:BBa_K260017|BBa_K260017]], with which it forms a functional unit depicted below: [anhydrotetracycline] -> [FLP] -> RFP
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[[Image:PTetFlp-F3-ZeoR-F3-TT-RFP.jpg]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 06:02, 20 October 2009

pTetFlp. [anhydrotetracycline] -> FLP(F70L)

This plasmid is based on pSC101 and contains TetR as well as the F70L variant of FLP recombinase under tet-promoter control. Therefore anhydrotetracycline can be used to induce FLP(F70L) expression.

The pSC101 origin of replication is temperature sensitive due to a mutation of repA. Permisive temperature is 30 degC. 37 degC should be avoided as the plasmid can not replicate.

This plasmid backbone is available with part BBa_K260017, with which it forms a functional unit depicted below: [anhydrotetracycline] -> [FLP] -> RFP PTetFlp-F3-ZeoR-F3-TT-RFP.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3222
    Illegal EcoRI site found at 5762
    Illegal XbaI site found at 67
    Illegal XbaI site found at 6532
    Illegal SpeI site found at 748
    Illegal SpeI site found at 5444
    Illegal PstI site found at 3132
    Illegal PstI site found at 4756
    Illegal PstI site found at 6283
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3222
    Illegal EcoRI site found at 5762
    Illegal EcoRI site found at 6680
    Illegal NheI site found at 1771
    Illegal SpeI site found at 2
    Illegal SpeI site found at 748
    Illegal SpeI site found at 5444
    Illegal PstI site found at 16
    Illegal PstI site found at 3132
    Illegal PstI site found at 4756
    Illegal PstI site found at 6283
    Illegal NotI site found at 9
    Illegal NotI site found at 6686
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3222
    Illegal EcoRI site found at 5762
    Illegal EcoRI site found at 6680
    Illegal BglII site found at 4969
    Illegal XhoI site found at 61
    Illegal XhoI site found at 6674
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 6680
    Illegal suffix found at 2
    Illegal EcoRI site found at 3222
    Illegal EcoRI site found at 5762
    Illegal XbaI site found at 67
    Illegal XbaI site found at 6532
    Illegal SpeI site found at 748
    Illegal SpeI site found at 5444
    Illegal PstI site found at 3132
    Illegal PstI site found at 4756
    Illegal PstI site found at 6283
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 6680
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3222
    Illegal EcoRI site found at 5762
    Illegal XbaI site found at 67
    Illegal XbaI site found at 6532
    Illegal XbaI site found at 6695
    Illegal SpeI site found at 2
    Illegal SpeI site found at 748
    Illegal SpeI site found at 5444
    Illegal PstI site found at 16
    Illegal PstI site found at 3132
    Illegal PstI site found at 4756
    Illegal PstI site found at 6283
    Illegal NgoMIV site found at 3927
    Illegal NgoMIV site found at 4305
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 4245
    Illegal SapI site found at 4455