Difference between revisions of "Part:BBa K4621003:Design"

 
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===Design Notes===
 
===Design Notes===
The specificity, effectiveness, GC content, off-targeting, and sgRNA self-complementation of the sgRNA will be taken into account when designing the part.
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The GC content will be taken into account when designing the primers.
 
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===Source===
 
===Source===
  
This part is derived from GQS52_08040 of the SCUT-3 genome.
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This part is derived from the region of the SCUT-3 genome immediately preceding GQS52_01520.
  
 
===References===
 
===References===

Latest revision as of 16:57, 1 October 2023


A whole functional sequence in front of the chitinase Chi19C gene, including its promoter.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 93
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The GC content will be taken into account when designing the primers.

Source

This part is derived from the region of the SCUT-3 genome immediately preceding GQS52_01520.

References