Difference between revisions of "Part:BBa K4765117"
m |
|||
| (10 intermediate revisions by 4 users not shown) | |||
| Line 3: | Line 3: | ||
<partinfo>BBa_K4765117 short</partinfo> | <partinfo>BBa_K4765117 short</partinfo> | ||
| − | + | <html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html> | |
| + | __TOC__ | ||
| + | |||
| + | ===Introduction=== | ||
| + | This composite part is composed of [https://parts.igem.org/Part:BBa_K4765112 BBa_K4765112] and [https://parts.igem.org/Part:BBa_K4765113 BBa_K4765113].The last stem-loop is replaced by T7 terminator. | ||
| + | ===Usage and Biology=== | ||
| + | We introduced this part into ''E. coli'', enabling the heterologous expression of both mtSSB and TDP proteins, to assess the combined desiccation resistance capability of these two proteins. | ||
| + | ===Characterization=== | ||
| + | ====Agarose gel electrophoresis==== | ||
| + | {| | ||
| + | | <html><img style="width:200px" src="https://static.igem.wiki/teams/4765/wiki/zsl/dna-gel/ssb-tdp.png" alt="contributed by Fudan iGEM 2023"></html> | ||
| + | |- | ||
| + | | '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures. | ||
| + | From left lane(1) to right lane(2) indicate the successful construction of ''H. ex'' mtSSB and ''H. ex'' mtSSB + SAHS 33020. ''' | ||
| + | |||
| + | |} | ||
| + | |||
| + | ====Successful Protein Expression==== | ||
| + | {| | ||
| + | | <html><img style="width:200px" src="https://static.igem.wiki/teams/4765/wiki/zsl/protein-gel/st.png" alt="contributed by Fudan iGEM 2023"></html> | ||
| + | |- | ||
| + | | '''Figure 2. SDS-PAGE electrophoresis of ribozyme connected: ''H. ex'' mtSSB + SAHS 33020''' | ||
| + | We constructed ribozyme connected: ''H. ex'' mtSSB + SAHS 33020 into the pET28a plasmid and transformed it into ''E. coli'' BL21 DE3. Lane 1 to 2 represent the IPTG uninduced and induced versions. As indicated by the red arrow, we successfully expressed ''H. ex'' mtSSB and SAHS 33020 in this part. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
| Line 9: | Line 31: | ||
<!-- --> | <!-- --> | ||
| − | + | ===Sequence and Features=== | |
<partinfo>BBa_K4765117 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4765117 SequenceAndFeatures</partinfo> | ||
| − | |||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Latest revision as of 15:53, 12 October 2023
ribozyme connected: H. ex mtSSB + SAHS 33020
Contents
Introduction
This composite part is composed of BBa_K4765112 and BBa_K4765113.The last stem-loop is replaced by T7 terminator.
Usage and Biology
We introduced this part into E. coli, enabling the heterologous expression of both mtSSB and TDP proteins, to assess the combined desiccation resistance capability of these two proteins.
Characterization
Agarose gel electrophoresis
|
| Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.
From left lane(1) to right lane(2) indicate the successful construction of H. ex mtSSB and H. ex mtSSB + SAHS 33020. |
Successful Protein Expression
|
| Figure 2. SDS-PAGE electrophoresis of ribozyme connected: H. ex mtSSB + SAHS 33020
We constructed ribozyme connected: H. ex mtSSB + SAHS 33020 into the pET28a plasmid and transformed it into E. coli BL21 DE3. Lane 1 to 2 represent the IPTG uninduced and induced versions. As indicated by the red arrow, we successfully expressed H. ex mtSSB and SAHS 33020 in this part. Sequence and Features
Assembly Compatibility:
|