Difference between revisions of "Part:BBa K4765116"
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===Introduction=== | ===Introduction=== | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
We constructed XRCC1 into pRAP to endow ''E. coli'' with anti-UV capability. | We constructed XRCC1 into pRAP to endow ''E. coli'' with anti-UV capability. | ||
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Latest revision as of 15:53, 12 October 2023
Twister P1 + T7_RBS + XRCC1 + stem-loop
Introduction
This composite part is composed of XRCC1 and ribozyme-assisted polycistronic co-expression system：pRAP. XRCC1 is a vital protein in DNA repair, particularly for single-strand breaks caused by radiation and alkylating agents.
Usage and Biology
We constructed XRCC1 into pRAP to endow E. coli with anti-UV capability.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 746
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1796