Difference between revisions of "Part:BBa K4765116"

 
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<partinfo>BBa_K4765116 short</partinfo>
 
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ribozyme+T7_RBS+XRCC1+stem-loop
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html>
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__TOC__
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===Introduction===
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This composite part is composed of XRCC1 and ribozyme-assisted polycistronic co-expression system：pRAP. XRCC1 is a vital protein in DNA repair, particularly for single-strand breaks caused by radiation and alkylating agents.
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===Usage and Biology===
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We constructed XRCC1 into pRAP to endow ''E. coli'' with anti-UV capability.
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4765116 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4765116 SequenceAndFeatures</partinfo>
  

Latest revision as of 15:53, 12 October 2023


Twister P1 + T7_RBS + XRCC1 + stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of XRCC1 and ribozyme-assisted polycistronic co-expression system：pRAP. XRCC1 is a vital protein in DNA repair, particularly for single-strand breaks caused by radiation and alkylating agents.

Usage and Biology

We constructed XRCC1 into pRAP to endow E. coli with anti-UV capability.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 746
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1796