Difference between revisions of "Part:BBa K4765113"
m |
|||
(9 intermediate revisions by 6 users not shown) | |||
Line 3: | Line 3: | ||
<partinfo>BBa_K4765113 short</partinfo> | <partinfo>BBa_K4765113 short</partinfo> | ||
− | ribozyme | + | <html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html> |
+ | __TOC__ | ||
+ | |||
+ | ===Introduction=== | ||
+ | This composite part is composed of ''Hypsibius exemplaris'' mitochondrial single-stranded DNA binding protein (''H. ex'' mtSSB) and improved ribozyme-assisted polycistronic co-expression system:pRAP. ''H. ex'' mtSSB can coat ssDNA to limit damage during desiccation and UV radiation. | ||
+ | |||
+ | ===Usage and Biology=== | ||
+ | We constructed SAHS 33020 into pRAP to endow ''E. coli'' with desiccation-resistant and anti-UV capability. And this composite part can cooperate with [https://parts.igem.org/Part:BBa_K4765112 BBa_K4765112] to increase the survival rate of ''E.coli'' under desiccation. | ||
+ | |||
+ | Get details in [https://parts.igem.org/Part:BBa_K4765016 BBa_K4765016]. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Line 9: | Line 18: | ||
<!-- --> | <!-- --> | ||
− | + | ===Sequence and Features=== | |
<partinfo>BBa_K4765113 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4765113 SequenceAndFeatures</partinfo> | ||
Latest revision as of 15:51, 12 October 2023
Twister P1 + T7_RBS + H. ex mtSSB + stem-loop
Introduction
This composite part is composed of Hypsibius exemplaris mitochondrial single-stranded DNA binding protein (H. ex mtSSB) and improved ribozyme-assisted polycistronic co-expression system:pRAP. H. ex mtSSB can coat ssDNA to limit damage during desiccation and UV radiation.
Usage and Biology
We constructed SAHS 33020 into pRAP to endow E. coli with desiccation-resistant and anti-UV capability. And this composite part can cooperate with BBa_K4765112 to increase the survival rate of E.coli under desiccation.
Get details in BBa_K4765016.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 247
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 515
Illegal BsaI.rc site found at 596
Illegal SapI site found at 409