Difference between revisions of "Part:BBa K4604005"
 
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Gene for bluB enzyme, catalyzes the reaction of FMNH2 to DMB used in the AdoCbl synthesis pathway in E. coli. Used in BioBricks...
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Gene for BluB enzyme, catalyzes the reaction of FMNH2 to DMB used in the AdoCbl synthesis pathway in <i>E. coli</i>. Ultimately leads to AdoCbl (B12) synthesis in cultures supplemented with cobinamide. Has a C-terminal his-tag. Also used in <a href="https://parts.igem.org/Part:BBa_K4604015">BBa_K4604015</a>, <a href="https://parts.igem.org/Part:BBa_K4604016">BBa_K4604016</a>, <a href="https://parts.igem.org/Part:BBa_K4604021">BBa_K4604021</a>, <a href="https://parts.igem.org/Part:BBa_K4604022">BBa_K4604022</a> and <a href="https://parts.igem.org/Part:BBa_K4604023">BBa_K4604023</a>. A more detailed characterization is uploaded on these respective registry pages. </html>
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<figure><center>
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<img src="https://static.igem.wiki/teams/4604/wiki/description/b12-production.png" width="700">
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<span style="font-size: smaller;"><b>Figure 1: Vitamin B12 production from precursors in <i>E. coli</i>.</b> The BluB transforms the reduced form of flavin mononucleotide (FMNH2) to form 5,6-dimethylbenzimidazole (DMB) which then joins exogenously-provided cobinamide to create vitamin B12. Made with biorender.com</span>
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===Usage and Biology===
 
  
 
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Latest revision as of 23:11, 11 October 2023


BluB

Gene for BluB enzyme, catalyzes the reaction of FMNH2 to DMB used in the AdoCbl synthesis pathway in E. coli. Ultimately leads to AdoCbl (B12) synthesis in cultures supplemented with cobinamide. Has a C-terminal his-tag. Also used in BBa_K4604015, BBa_K4604016, BBa_K4604021, BBa_K4604022 and BBa_K4604023. A more detailed characterization is uploaded on these respective registry pages.


Figure 1: Vitamin B12 production from precursors in E. coli. The BluB transforms the reduced form of flavin mononucleotide (FMNH2) to form 5,6-dimethylbenzimidazole (DMB) which then joins exogenously-provided cobinamide to create vitamin B12. Made with biorender.com



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]