Difference between revisions of "Part:BBa K260001:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K260001 short</partinfo>
 
<partinfo>BBa_K260001 short</partinfo>
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===Source===
 
===Source===
  
−
Based on BBa_K260000, so it also contains human MECP2 genomic sequence, but has an insert taken from BBa_K260010.
+
Based on [[Part:BBa_K260000|BBa_K260000]], so it also contains human MECP2 genomic sequence, but has an insert taken from [[Part:BBa_K260010|BBa_K260010]].
  
 
===References===
 
===References===

Latest revision as of 17:53, 17 October 2009

pCC2FosMB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1738
    Illegal EcoRI site found at 2324
    Illegal EcoRI site found at 3809
    Illegal EcoRI site found at 4889
    Illegal EcoRI site found at 14477
    Illegal EcoRI site found at 21238
    Illegal EcoRI site found at 30506
    Illegal XbaI site found at 7175
    Illegal XbaI site found at 10053
    Illegal XbaI site found at 14642
    Illegal XbaI site found at 14930
    Illegal XbaI site found at 17884
    Illegal XbaI site found at 17915
    Illegal XbaI site found at 20234
    Illegal SpeI site found at 2573
    Illegal SpeI site found at 28757
    Illegal PstI site found at 1836
    Illegal PstI site found at 1968
    Illegal PstI site found at 2697
    Illegal PstI site found at 4323
    Illegal PstI site found at 9689
    Illegal PstI site found at 10032
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1738
    Illegal EcoRI site found at 2324
    Illegal EcoRI site found at 3809
    Illegal EcoRI site found at 4889
    Illegal EcoRI site found at 14477
    Illegal EcoRI site found at 21238
    Illegal EcoRI site found at 30506
    Illegal NheI site found at 17298
    Illegal NheI site found at 48089
    Illegal SpeI site found at 2
    Illegal SpeI site found at 2573
    Illegal SpeI site found at 28757
    Illegal PstI site found at 16
    Illegal PstI site found at 1836
    Illegal PstI site found at 1968
    Illegal PstI site found at 2697
    Illegal PstI site found at 4323
    Illegal PstI site found at 9689
    Illegal PstI site found at 10032
    Illegal NotI site found at 9
    Illegal NotI site found at 22677
    Illegal NotI site found at 30175
    Illegal NotI site found at 48157
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1738
    Illegal EcoRI site found at 2324
    Illegal EcoRI site found at 3809
    Illegal EcoRI site found at 4889
    Illegal EcoRI site found at 14477
    Illegal EcoRI site found at 21238
    Illegal EcoRI site found at 30506
    Illegal BglII site found at 2335
    Illegal BglII site found at 25181
    Illegal BglII site found at 27248
    Illegal BglII site found at 37967
    Illegal BglII site found at 39393
    Illegal BglII site found at 41566
    Illegal BglII site found at 46713
    Illegal BamHI site found at 1870
    Illegal BamHI site found at 4280
    Illegal BamHI site found at 22400
    Illegal BamHI site found at 30527
    Illegal BamHI site found at 31427
    Illegal BamHI site found at 31887
    Illegal BamHI site found at 47143
    Illegal XhoI site found at 4046
    Illegal XhoI site found at 31812
    Illegal XhoI site found at 36045
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 48151
    Illegal suffix found at 2
    Illegal EcoRI site found at 1738
    Illegal EcoRI site found at 2324
    Illegal EcoRI site found at 3809
    Illegal EcoRI site found at 4889
    Illegal EcoRI site found at 14477
    Illegal EcoRI site found at 21238
    Illegal EcoRI site found at 30506
    Illegal XbaI site found at 7175
    Illegal XbaI site found at 10053
    Illegal XbaI site found at 14642
    Illegal XbaI site found at 14930
    Illegal XbaI site found at 17884
    Illegal XbaI site found at 17915
    Illegal XbaI site found at 20234
    Illegal SpeI site found at 2573
    Illegal SpeI site found at 28757
    Illegal PstI site found at 1836
    Illegal PstI site found at 1968
    Illegal PstI site found at 2697
    Illegal PstI site found at 4323
    Illegal PstI site found at 9689
    Illegal PstI site found at 10032
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 48151
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1738
    Illegal EcoRI site found at 2324
    Illegal EcoRI site found at 3809
    Illegal EcoRI site found at 4889
    Illegal EcoRI site found at 14477
    Illegal EcoRI site found at 21238
    Illegal EcoRI site found at 30506
    Illegal XbaI site found at 7175
    Illegal XbaI site found at 10053
    Illegal XbaI site found at 14642
    Illegal XbaI site found at 14930
    Illegal XbaI site found at 17884
    Illegal XbaI site found at 17915
    Illegal XbaI site found at 20234
    Illegal SpeI site found at 2
    Illegal SpeI site found at 2573
    Illegal SpeI site found at 28757
    Illegal PstI site found at 16
    Illegal PstI site found at 1836
    Illegal PstI site found at 1968
    Illegal PstI site found at 2697
    Illegal PstI site found at 4323
    Illegal PstI site found at 9689
    Illegal PstI site found at 10032
    Illegal NgoMIV site found at 12865
    Illegal NgoMIV site found at 13913
    Illegal NgoMIV site found at 24520
    Illegal NgoMIV site found at 24527
    Illegal NgoMIV site found at 24651
    Illegal NgoMIV site found at 25738
    Illegal NgoMIV site found at 29654
    Illegal AgeI site found at 8570
    Illegal AgeI site found at 13992
    Illegal AgeI site found at 25862
    Illegal AgeI site found at 27092
    Illegal AgeI site found at 27985
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 3602
    Illegal BsaI site found at 4043
    Illegal BsaI site found at 5783
    Illegal BsaI site found at 6346
    Illegal BsaI site found at 6934
    Illegal BsaI site found at 8864
    Illegal BsaI site found at 15098
    Illegal BsaI.rc site found at 675
    Illegal BsaI.rc site found at 1911
    Illegal BsaI.rc site found at 11284
    Illegal BsaI.rc site found at 11472
    Illegal BsaI.rc site found at 12748
    Illegal BsaI.rc site found at 14199
    Illegal BsaI.rc site found at 20602
    Illegal SapI site found at 6462
    Illegal SapI site found at 13800
    Illegal SapI site found at 16311
    Illegal SapI site found at 16333
    Illegal SapI site found at 26631
    Illegal SapI site found at 27841
    Illegal SapI.rc site found at 18502
    Illegal SapI.rc site found at 20759
    Illegal SapI.rc site found at 46761


Design Notes

The insertion site for BsdR was chosen to be upstream of MECP2 exon 1 but still with more than 10 kb of MECP genomic sequence upstream.


Source

Based on BBa_K260000, so it also contains human MECP2 genomic sequence, but has an insert taken from BBa_K260010.

References