Difference between revisions of "Part:BBa K4789004:Design"
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[1] Chen Xianjun,Zhang Dasheng,Su Ni et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs.[J] .Nat Biotechnol, 2019, 37: 1287-1293. | [1] Chen Xianjun,Zhang Dasheng,Su Ni et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs.[J] .Nat Biotechnol, 2019, 37: 1287-1293. | ||
Latest revision as of 07:48, 30 September 2023
miR-22-sponge-pepper
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 867
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 867
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 867
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 867
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We use the “Pepper ” to visualize LncRNA MALAT1 in order to reflect miRNA expression in vivo. The pepper-MALAT1 plasmid will emit green fluorescence when HBC is added. In the healthy people, there were fewer miR-22. So fewer miRNA could bind to MALAT1.More free MALAT1 will emit fluorescence through reporter “Pepper assay”.
Source
The plasmid of pCDNA3-MCS-nPepper was obtained from Naying Biotechnology Company.
References
[1] Chen Xianjun,Zhang Dasheng,Su Ni et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs.[J] .Nat Biotechnol, 2019, 37: 1287-1293.