Difference between revisions of "Part:BBa K4674000:Design"

 
(→‎References)
 
(One intermediate revision by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
To express the mGL in E.Coli, we codon optimized the mGL sequence.
+
The sequence of mGL is codon optimized for E. Coli expression
−
This part fits RCF10 standard.
+
−
 
+
−
 
+
  
 
===Source===
 
===Source===
Line 17: Line 14:
  
 
===References===
 
===References===
 +
mGreenLantern: a bright monomeric fluorescent protein with rapid expression and cell filling properties for neuronal imaging
 +
Campbell Bc, Nabel Em, Murdock Mh, Lao-Peregrin C, Tsoulfas P, Blackmore Mg, Lee Fs, Liston C, Morishita H, Petsko Ga
 +
 +
(2020). Proceedings of the National Academy of Sciences, , 202000942. doi: 10.1073/pnas.2000942117.

Latest revision as of 02:34, 4 October 2023


mGreenLantern (mGL)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16


Design Notes

The sequence of mGL is codon optimized for E. Coli expression

Source

mGreenLantern (mGL) is derived from Aequorea victoria

References

mGreenLantern: a bright monomeric fluorescent protein with rapid expression and cell filling properties for neuronal imaging Campbell Bc, Nabel Em, Murdock Mh, Lao-Peregrin C, Tsoulfas P, Blackmore Mg, Lee Fs, Liston C, Morishita H, Petsko Ga

(2020). Proceedings of the National Academy of Sciences, , 202000942. doi: 10.1073/pnas.2000942117.