Difference between revisions of "Part:BBa K4885010"

Latest revision as of 05:49, 10 October 2023

10

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
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COMPATIBLE WITH RFC[12]
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COMPATIBLE WITH RFC[21]
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COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4885010 short</partinfo>
-This part is responsible for the expression of adhE2, NGbcd (BBa_K4885007) and NGcrt (BBa_K4885008) with Pthl promoter. adhE2 gene is derived from Clostridium acetobutylicum and codes for the alcohol/aldehyde bifunctional dehydrogenase. NGbcd contains NGCatcher sequence and bcd gene and codes for fusion protein of NGCatcher and butyryl-CoA dehydrogenase. NGcrt contains NGTag sequence and crt gene and codes for NGTag-crotonase fusion protein. Bcd and crt genes are derived from Clostridium tyrobutyricum (C. tyrobutyricum) L319.
-Alcohol/aldehyde bifunctional dehydrogenase converts acyl-CoA to aldehyde and then to alcohol in two reductive steps. Crotonase converts 3-hydroxybutyryl-CoA to crotonyl-CoA, and butyryl-CoA dehydrogenase converts crotonyl-CoA to butyryl-CoA in the butyrate synthesis pathway in C. tyrobutyricum. NGTag forms a covalent bond with NGCatcher rapidly which is not affected by the change of environment. Butyryl-CoA dehydrogenase (bcd) and crotonase (crt) modified by NGCatcher/NGTag oligopeptides can be assembled together. Pthl is a strong transcriptional promoter in C. tyrobutyricum.
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 <partinfo>BBa_K4885010 SequenceAndFeatures</partinfo>
-==Results==
-===(1)Plasmid construction and transformation===
-===(2)Product yields of the engineered C. tyrobutyricum ===
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 ===Functional Parameters===
 <partinfo>BBa_K4885010 parameters</partinfo>
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