Difference between revisions of "Part:BBa K4885008:Design"

Latest revision as of 04:23, 10 October 2023

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Assembly Compatibility:

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 ===Design Notes===
-In the butyrate synthesis pathway of C. tyrobutyricum L319,  crotonase (crt) converts 3-hydroxybutyryl-CoA to crotonyl-CoA which is subsequently converted to butyryl-CoA by butyryl-CoA dehydrogenase coded by bcd. These two enzymes are rate-limiting enzymes.
-We used this part and BBa_K4885007 to express fusion protein of NGTag and crotonase (crt) and fusion protein of NGCatcher and butyryl-CoA dehydrogenase (bcd) in C. tyrobutyricum, which allows the assembly of crotonase and butyryl-CoA dehydrogenase via the NGTag/NGCatcher peptide interaction. In this way, we were able to enhance the butyrate synthesis pathway in the strain.
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 ===Source===
-NGTag sequence is from  BBa_K4119030
-crt sequence is from BBa_K4119028.
 ===References===