Difference between revisions of "Part:BBa K4886004:Design"
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− | <img class="bild" src="https://static.igem.wiki/teams/4886/wiki/nog-pathway.png"> | + | <img class="bild" src="https://static.igem.wiki/teams/4886/wiki/parts/nog-pathway.png"> |
<div class="unterschrift"><b>Figure 1 NOG pathway and related enzyme genes (red arrows indicate lack of key enzymes in Clostridium tyrobutyricum)</b> | <div class="unterschrift"><b>Figure 1 NOG pathway and related enzyme genes (red arrows indicate lack of key enzymes in Clostridium tyrobutyricum)</b> | ||
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===Source=== | ===Source=== | ||
− | The Pfba promoter sequence is from BBa_K4886003. | + | <p>The Pfba promoter sequence is from BBa_K4886003. </p> |
− | The strong RBS sequence is from BBa_K103015. | + | <p>The strong RBS sequence is from BBa_K103015.</p> |
− | The F/Xpk sequence is from BBa_K4119076. | + | <p>The F/Xpk sequence is from BBa_K4119076.</p> |
− | The terminator sequence is from BBa_K3585002. | + | <p>The terminator sequence is from BBa_K3585002.</p> |
===References=== | ===References=== |
Latest revision as of 16:19, 11 October 2023
Pfba-FXpk(BD)
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1689
Illegal XbaI site found at 632 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1689
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1689
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1689
Illegal XbaI site found at 632 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1689
Illegal XbaI site found at 632 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Our project aims to reduce the carbon emission during the fermentation of Clostridium tyrobutyricum (C. tyrobutyricum) by constructing a NOG pathway to integrate with the native EMP pathway of the strain. The NOG pathway is an artificial pathway and functions as a fructose-6-phosphate (F6P) shunt. Through carbon rearrangement, this pathway can convert 1mol of F6P to 3mol of AcP without any loss of carbon. By comparison, we found that most of the key enzymes in the NOG pathway natively exist in C. tyrobutyricum and the functions of the absent key enzymes can be carried out by phosphoketolases (Figure 1). Therefore, in order to construct the NOG pathway in C. tyrobutyricum L319, we introduced phosphoketolase (F/Xpk) gene into the strain. Pthl promoter is a strong transcriptional promoter in C. tyrobutyricum L319. We used this promoter to ensure a robust expression in the strain.
Source
The Pfba promoter sequence is from BBa_K4886003.
The strong RBS sequence is from BBa_K103015.
The F/Xpk sequence is from BBa_K4119076.
The terminator sequence is from BBa_K3585002.