Difference between revisions of "Part:BBa K4765007"
(→Introduction) |
|||
(8 intermediate revisions by 3 users not shown) | |||
Line 1: | Line 1: | ||
+ | __NOTOC__ | ||
+ | <partinfo>BBa_K4765007 short</partinfo> | ||
+ | <html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html> | ||
+ | __TOC__ | ||
+ | ===Introduction=== | ||
+ | Nb3 is a variant of nanobody, a type of single-domain antibody<ref>Glass, D. S., & Riedel-Kruse, I. H. (2018). A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. ''Cell, 174''(3), 649-658.e16. https://doi.org/10.1016/j.cell.2018.06.041 | ||
+ | </ref> | ||
+ | . It can interact with [https://parts.igem.org/Part:BBa_K4765006 BBa_K4765006(Ag3)] and the interaction between Ag-Nb can mediate specific binding between ''E.coli''. | ||
+ | |||
+ | ===Usage and Biology=== | ||
+ | The combination of Nb3's single-domain structure and the intimin surface display system allows the entirety of a highly specific, cell surface-bound adhesin to be encoded as a single fusion protein. | ||
+ | |||
+ | ===Characterization=== | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K4765007 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K4765007 parameters</partinfo> | ||
+ | <!-- --> | ||
+ | ==Reference== |
Latest revision as of 15:15, 12 October 2023
Nb3, R4P43 from 10.1016/j.cell.2018.06.041
Introduction
Nb3 is a variant of nanobody, a type of single-domain antibody[1] . It can interact with BBa_K4765006(Ag3) and the interaction between Ag-Nb can mediate specific binding between E.coli.
Usage and Biology
The combination of Nb3's single-domain structure and the intimin surface display system allows the entirety of a highly specific, cell surface-bound adhesin to be encoded as a single fusion protein.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 166
Reference
- ↑ Glass, D. S., & Riedel-Kruse, I. H. (2018). A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. Cell, 174(3), 649-658.e16. https://doi.org/10.1016/j.cell.2018.06.041