Difference between revisions of "Part:BBa K4814010"
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We have used the codon optimized version of EYFP by Watcher, R. M. et al (1998). This protein is used to construct a FRET pair to detect DNA damage in mammalian cells. Please refer to BBa_K4814009. | We have used the codon optimized version of EYFP by Watcher, R. M. et al (1998). This protein is used to construct a FRET pair to detect DNA damage in mammalian cells. Please refer to BBa_K4814009. | ||
The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. | The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. | ||
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| + | Click here for the experiment data: <html><a href="https://parts.igem.org/Part:BBa_K4814009">RPA1-EYFP BBa_K4814009</a></html> | ||
References: | References: | ||
Wachter, R. M., Elsliger, M. A., Kallio, K., Hanson, G. T., & Remington, S. J. (1998). Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein. Structure (London, England : 1993), 6(10), 1267–1277. https://doi.org/10.1016/s0969-2126(98)00127-0 | Wachter, R. M., Elsliger, M. A., Kallio, K., Hanson, G. T., & Remington, S. J. (1998). Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein. Structure (London, England : 1993), 6(10), 1267–1277. https://doi.org/10.1016/s0969-2126(98)00127-0 | ||
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| + | Sequence derived from: https://www.snapgene.com/plasmids/fluorescent_protein_genes_and_plasmids/EYFP | ||
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Latest revision as of 09:52, 12 October 2023
Mammalian Codon Optimized EYFP
We have used the codon optimized version of EYFP by Watcher, R. M. et al (1998). This protein is used to construct a FRET pair to detect DNA damage in mammalian cells. Please refer to BBa_K4814009.
The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date.
Click here for the experiment data: RPA1-EYFP BBa_K4814009
References:
Wachter, R. M., Elsliger, M. A., Kallio, K., Hanson, G. T., & Remington, S. J. (1998). Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein. Structure (London, England : 1993), 6(10), 1267–1277. https://doi.org/10.1016/s0969-2126(98)00127-0
Sequence derived from: https://www.snapgene.com/plasmids/fluorescent_protein_genes_and_plasmids/EYFP
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]