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A protein of truncated gonadotropin-releasing hormone.
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A protein of truncated gonadotropin-releasing hormone.GnRH secreted by the hypothalamus plays an important role in the regulation of vertebrate reproduction. It is a peptide of 10 amino acids, which can be synthesized artificially. It can release luteinizing hormone and follicle stimulating hormone. It is mainly distributed in the lateral area of median eminence, as well as in arcuate nucleus, preoptic area of hypothalamus, ependymal cells and pineal gland.
 
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                 Insulin
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                 1.Diagrams
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                <p>We have proved that Insulin can be processed and secreted outside the cell in 293T cells.
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                1 diagram
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                     <img width="400px" src="https://static.igem.wiki/teams/4585/wiki/gnrh.png"></p>
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                     <img width="400px" src="https://static.igem.wiki/teams/4585/wiki/gnrh-cmoshitu.png"></p>
 
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                 <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram of 9XUAS-Insulin</p>
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                 <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram of GnRH-C</p>
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                2.Experiment
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                2.1 Method
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                <p>In the experiment, we used an ELIAS kit specially designed to detect human mature insulin to detect cell eliquidation.
 
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                2.2 Results
 
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                <p>Insulin can be processed and secreted outside the cell in 293T cells.
 
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                     <img width="400px" src="https://2021.igem.org/wiki/images/5/58/T--CSU_CHINA--tupian30.png"></p>
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                     <img width="400px" src="https://static.igem.wiki/teams/4585/wiki/gnrh-c.png"></p>
 
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                 <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.2 Standard curve of absorbance and insulin concentration</p>
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                 <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.2 Agarose gel electrophoresis map of GnRH-C fragment PCR product</p>
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                    <img width="400px" src="https://2021.igem.org/wiki/images/5/57/T--CSU_CHINA--tupian31.png"></p>
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.3 Insulin concentration in supernatant after blue light irradiation and dark treatment respectively</p>
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.4 Insulin concentration in supernatant (excluding insulin in medium) after blue light irradiation and dark treatment respectively</p>
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                 3.Caution
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                2.Caution
 
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                 <p>Insulin is not suitable for long time preservation under the best culture temperature of cells (37℃). In the mean time, cells would also consume some of the expressed insulin. So the best tst time for Insulin is approximately 24 hours
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                 <p>GnRH-C is the result of truncation of full-length GnRH.
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                    after transfection, it is relatively shorter than the test interval of LUC.
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                Reference：
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                <p>[1]Mingqi Xie, Haifeng Ye, Hui Wang.β-cell-mimetic designer cells provide closed-loop glycemic control[J].Science.2016 Dec 9;354(6317):1296-1301.
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Latest revision as of 02:14, 9 October 2023


GnRH-C

A protein of truncated gonadotropin-releasing hormone.GnRH secreted by the hypothalamus plays an important role in the regulation of vertebrate reproduction. It is a peptide of 10 amino acids, which can be synthesized artificially. It can release luteinizing hormone and follicle stimulating hormone. It is mainly distributed in the lateral area of median eminence, as well as in arcuate nucleus, preoptic area of hypothalamus, ependymal cells and pineal gland.

1.Diagrams

Fig.1 The model diagram of GnRH-C

Fig.2 Agarose gel electrophoresis map of GnRH-C fragment PCR product

2.Caution

GnRH-C is the result of truncation of full-length GnRH.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]