Difference between revisions of "Part:BBa K4642013"

 
 
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This composite part is used for testing the presence of hsa-miR-145, hsa-miR-199a and hsa-miR-125b. It has a T7 promoter for expression of functional RNA BBa_K1614000. This is followed by the code for our AND-gate toehold switch BBa_K4642005 and the anti-miRNAs BBa_K4642006 and BBa_K4642007. Placing a reporter protein downstream of this toehold switch part will mean that when our trigger RNAs are present in high enough quantities, the protein in the CDS will be translated and the reporter protein can be observed. Our chosen reporter protein is mCherry BBa_J06504. This fluorescence produced can be detected with a luminometer to affirm a positive test.
 
This composite part is used for testing the presence of hsa-miR-145, hsa-miR-199a and hsa-miR-125b. It has a T7 promoter for expression of functional RNA BBa_K1614000. This is followed by the code for our AND-gate toehold switch BBa_K4642005 and the anti-miRNAs BBa_K4642006 and BBa_K4642007. Placing a reporter protein downstream of this toehold switch part will mean that when our trigger RNAs are present in high enough quantities, the protein in the CDS will be translated and the reporter protein can be observed. Our chosen reporter protein is mCherry BBa_J06504. This fluorescence produced can be detected with a luminometer to affirm a positive test.

Latest revision as of 14:54, 11 October 2023


T7 / Gen4 145-199a-125b Double AND-Gate / mCherry / Double Terminator / T7 / Gen4 145-199a-125b Doub

This composite part is used for testing the presence of hsa-miR-145, hsa-miR-199a and hsa-miR-125b. It has a T7 promoter for expression of functional RNA BBa_K1614000. This is followed by the code for our AND-gate toehold switch BBa_K4642005 and the anti-miRNAs BBa_K4642006 and BBa_K4642007. Placing a reporter protein downstream of this toehold switch part will mean that when our trigger RNAs are present in high enough quantities, the protein in the CDS will be translated and the reporter protein can be observed. Our chosen reporter protein is mCherry BBa_J06504. This fluorescence produced can be detected with a luminometer to affirm a positive test.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 62
    Illegal BsaI.rc site found at 106