Difference between revisions of "Part:BBa K4814010"

 
 
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__NOTOC__
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<partinfo>BBa_K4814010 short</partinfo>
  
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We have used the codon optimized version of EYFP by Watcher, R. M. et al (1998). This protein is used to construct a FRET pair to detect DNA damage in mammalian cells. Please refer to BBa_K4814009.
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The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date.
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Click here for the experiment data: <html><a href="https://parts.igem.org/Part:BBa_K4814009">RPA1-EYFP BBa_K4814009</a></html>
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References:
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Wachter, R. M., Elsliger, M. A., Kallio, K., Hanson, G. T., & Remington, S. J. (1998). Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein. Structure (London, England : 1993), 6(10), 1267–1277. https://doi.org/10.1016/s0969-2126(98)00127-0
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Sequence derived from: https://www.snapgene.com/plasmids/fluorescent_protein_genes_and_plasmids/EYFP
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4814010 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K4814010 parameters</partinfo>
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Latest revision as of 09:52, 12 October 2023

Mammalian Codon Optimized EYFP


We have used the codon optimized version of EYFP by Watcher, R. M. et al (1998). This protein is used to construct a FRET pair to detect DNA damage in mammalian cells. Please refer to BBa_K4814009.

The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date.

Click here for the experiment data: RPA1-EYFP BBa_K4814009

References:

Wachter, R. M., Elsliger, M. A., Kallio, K., Hanson, G. T., & Remington, S. J. (1998). Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein. Structure (London, England : 1993), 6(10), 1267–1277. https://doi.org/10.1016/s0969-2126(98)00127-0

Sequence derived from: https://www.snapgene.com/plasmids/fluorescent_protein_genes_and_plasmids/EYFP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]