Difference between revisions of "Part:BBa K4937024"

Latest revision as of 11:27, 3 October 2023

TDH3p-SPE2-DIT1t

This part is used to construct the plasmid library that can stably express SPE2 and tune the expression level of SPE3 and AtACL5 to fully use the spermidine substrate and examine the numerical relationship between spermidine and thermospermine.

We constructed this part by two round PCR. We firstly achieved all short single fragment by PCR from the genome of S. cerevisiae and plasmid (Figure 1). Then we connected all single expression device by fusion PCR (Figure 2).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4937024 short</partinfo>
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+<p>This part is used to construct the plasmid library that can stably express SPE2 and tune the expression level of SPE3 and AtACL5 to fully use the spermidine substrate and examine the numerical relationship between spermidine and thermospermine.</p>
+https://static.igem.wiki/teams/4937/wiki/part/24-1.png
+<p>We constructed this part by two round PCR. We firstly achieved all short single fragment by PCR from the genome of <i>S. cerevisiae</i> and plasmid (Figure 1). Then we connected all single expression device by fusion PCR (Figure 2). </p>
+https://static.igem.wiki/teams/4937/wiki/part/24-2.png
+https://static.igem.wiki/teams/4937/wiki/part/24-3.png
 <!-- Add more about the biology of this part here
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-<span class='h3bb'>Sequence and Features</span>
+===Sequence and Features===
 <partinfo>BBa_K4937024 SequenceAndFeatures</partinfo>