Difference between revisions of "Part:BBa K4585005"

 
 
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<partinfo>BBa_K4585005 short</partinfo>
 
<partinfo>BBa_K4585005 short</partinfo>
  
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A degradation signal formed by several amino acid residues at the end of a protein.
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Degron protein degradation sequence. Degron is a short peptide with a length of 41 amino acids that binds to E3 ubiquitin ligase through a disaster-to-order conversion, causing the substrate to be ubiquitinated and subsequently degraded.
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<head>
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</head>
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<body>
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            <h2 class="pageContent-main__title pageContent-main__subtitle">
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                <!--class="pageContent-main__title" means it is the sub title-->
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                Diagrams
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            </h2>
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            <div class="pageContent-main__textBox">
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                <!--all the content must included in this div-->
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                <p style="text-align: center;">
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                    <img width="400px" src="https://static.igem.wiki/teams/4585/wiki/the-model-diagram-of-pcdna3-1-3-ha-gal4-vp64-degron-nls111.png"></p>
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                <p style="text-align: center;">
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                    <img width="400px" src="https://static.igem.wiki/teams/4585/wiki/the-model-diagram-of-pgl4-35-3-ha-9-gal4uas-krab-degron-nls111.png"></p>
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<br />
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig 1. The model diagram of Degron</p>
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                <p style="text-align: center;">
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                    <img width="400px" src="https://static.igem.wiki/teams/4585/wiki/fig-2-the-agarose-gel-electrophoresis-of-degron-1.png"></p>
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                <p style="text-align: center;">
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                    <img width="400px" src="https://static.igem.wiki/teams/4585/wiki/fig-2-the-agarose-gel-electrophoresis-of-degron-2.png"></p>
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig 2. The agarose gel electrophoresis maps of Degron fragment PCR products</p>
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            </div>
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</body>
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</html>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 03:27, 9 October 2023

Degron

Degron protein degradation sequence. Degron is a short peptide with a length of 41 amino acids that binds to E3 ubiquitin ligase through a disaster-to-order conversion, causing the substrate to be ubiquitinated and subsequently degraded.

Diagrams


Fig 1. The model diagram of Degron


Fig 2. The agarose gel electrophoresis maps of Degron fragment PCR products


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 92
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 92
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 92
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 92
  • 1000
    COMPATIBLE WITH RFC[1000]