Difference between revisions of "Part:BBa K4601051"

 
 
(6 intermediate revisions by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K4601051 short</partinfo>
 
<partinfo>BBa_K4601051 short</partinfo>
  
−
to do
+
This part is CmR gene coding for the chloramphenicol ''O''-acetyltransferase enzyme (Uniprot P62577).
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
The chloramphenicol ''O''-acetyltransferase enzyme (EC 2.3.1.28) catalyses the conversion of chloramphenicol into chloramphenicol 3-acetate (Figure 1), thus inactivating the antibiotic.
 +
 +
<html>
 +
<img src="https://static.igem.wiki/teams/4601/wiki/parts-registry/t-evry-paris-saclay-cmr-reaction.png" width="500px">
 +
</html>
 +
 +
Figure 1. The mode of action of the chloramphenicol ''O''-acetyltransferase enzyme: acetylation of chloramphenicol.
 +
 +
The CmR gene was isolated from the bacteriophage P1Cm carrying the Tn''cam''-204 transposon derived from the R plasmid NR1 [1].
 +
 +
 +
===Reference===
 +
[1] Marcoli R, Iida S, Bickle TA. The DNA sequence of an IS/-flanked transposon coding for resistance to chloramphenicol and fusidic acid. FEBS letters (1980) 110: 11–14.
  
 
<!-- -->
 
<!-- -->

Latest revision as of 12:58, 3 October 2023


CmR

This part is CmR gene coding for the chloramphenicol O-acetyltransferase enzyme (Uniprot P62577).

Usage and Biology

The chloramphenicol O-acetyltransferase enzyme (EC 2.3.1.28) catalyses the conversion of chloramphenicol into chloramphenicol 3-acetate (Figure 1), thus inactivating the antibiotic.

Figure 1. The mode of action of the chloramphenicol O-acetyltransferase enzyme: acetylation of chloramphenicol.

The CmR gene was isolated from the bacteriophage P1Cm carrying the Tncam-204 transposon derived from the R plasmid NR1 [1].


Reference

[1] Marcoli R, Iida S, Bickle TA. The DNA sequence of an IS/-flanked transposon coding for resistance to chloramphenicol and fusidic acid. FEBS letters (1980) 110: 11–14.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]