Difference between revisions of "Part:BBa K185002"
 
Line 2: Line 2:
 
<partinfo>BBa_K185002 short</partinfo>
 
<partinfo>BBa_K185002 short</partinfo>
  
−
The Lon protease is conneced by double terminator BBa_B0015.Take attention that this part contains a PstI restriction site. So if someone wants to use this part, it is better to cut it with SpeI in the suufix region.
+
The Lon protease is conneced by double terminator BBa_B0015.Take attention that this part contains a PstI restriction site. So if someone wants to use this part, it is better to cut it with SpeI in the suffix region.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 07:13, 21 October 2009

Lon Protease+double terminater

The Lon protease is conneced by double terminator BBa_B0015.Take attention that this part contains a PstI restriction site. So if someone wants to use this part, it is better to cut it with SpeI in the suffix region.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 619
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 619
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1332
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 619
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 619
    Illegal AgeI site found at 1795
    Illegal AgeI site found at 1879
    Illegal AgeI site found at 2077
    Illegal AgeI site found at 2110
  • 1000
    COMPATIBLE WITH RFC[1000]