Difference between revisions of "Part:BBa K4585002"
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<partinfo>BBa_K4585002 short</partinfo> | <partinfo>BBa_K4585002 short</partinfo> | ||
− | VP64 is a kind of protein that can activate downstream gene expression. | + | VP64 is a kind of protein that can activate downstream gene expression. It is a transcription activation factor which can realize the activation of downstream UAS transcription. |
+ | <html> | ||
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+ | <head> | ||
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+ | </head> | ||
+ | |||
+ | <body> | ||
+ | <h2 class="pageContent-main__title"> | ||
+ | <!--put tile here, <h2>title</h2>, class="pageContent-main__title" means it is the main title--> | ||
+ | |||
+ | 1.Diagrams | ||
+ | </h2> | ||
+ | <div class="pageContent-main__textBox"> | ||
+ | <!--all the content must included in this div--> | ||
+ | <p style="text-align: center;"> | ||
+ | <img width="400px" src="https://static.igem.wiki/teams/4585/wiki/vp64moshit.png"></p> | ||
+ | <!--put image's url here--> | ||
+ | <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram of VP64</p> | ||
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+ | |||
+ | </h2> | ||
+ | <div class="pageContent-main__textBox"> | ||
+ | <!--all the content must included in this div--> | ||
+ | <p style="text-align: center;"> | ||
+ | <img width="400px" src="https://static.igem.wiki/teams/4585/wiki/gal4-vp64.png | ||
+ | "></p> | ||
+ | <!--put image's url here--> | ||
+ | <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.2 Agarose gel electrophoresis map of VP64 fragment PCR product</p> | ||
+ | </div> | ||
+ | <h2 class="pageContent-main__title pageContent-main__subtitle"> | ||
+ | <!--class="pageContent-main__title" means it is the sub title--> | ||
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+ | 2.Caution | ||
+ | </h2> | ||
+ | <div class="pageContent-main__textBox"> | ||
+ | <!--all the content must included in this div--> | ||
+ | <p>GAL4 combining domain is universal among eukaryotic cells, but the activation factor needs to be chosen differently according to the chassis creature. | ||
+ | </p> | ||
+ | </div> | ||
+ | <h2 class="pageContent-main__title pageContent-main__subtitle"> | ||
+ | <!--class="pageContent-main__title" means it is the sub title--> | ||
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+ | </body> | ||
+ | </html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 02:04, 9 October 2023
VP64
VP64 is a kind of protein that can activate downstream gene expression. It is a transcription activation factor which can realize the activation of downstream UAS transcription.
1.Diagrams
Fig.1 The model diagram of VP64
Fig.2 Agarose gel electrophoresis map of VP64 fragment PCR product
2.Caution
GAL4 combining domain is universal among eukaryotic cells, but the activation factor needs to be chosen differently according to the chassis creature.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 138
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 138
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 138
- 1000COMPATIBLE WITH RFC[1000]