Difference between revisions of "Part:BBa K4606004:Design"
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===Source=== | ===Source=== | ||
− | + | The base sequences were derived from the database of Targetscan website. | |
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===References=== | ===References=== |
Latest revision as of 02:05, 23 September 2023
SMAD4(mutant type)
For detailed design information, you can look up the registry of (BBa_K 4606003), the device for gene segment of SMAD4. According to the base sequence information of SMAD4 in the database, we mutated the SMAD4 gene in a region that complementary binds to miR-3074-5p. By inserting its 3’UTR region (414bp) behind the luciferase gene in the vector, we could determine the expression level of this gene segment.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Based on the online repository of miRNA targets, SMAD4 might be a target of miR-3074-5p in osteoarthritis. Here we mutated the SMAD4 gene (wild type) in a region that complementary binds to miR-3074-5p.
Source
The base sequences were derived from the database of Targetscan website.