Difference between revisions of "Part:BBa K283029"
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| + | This part used to construct a device which can generate repressor of pTet regulator consitutively. TetR repression is inhibited by the addition of tetracycline or its analog, aTc. We inserted ''cheZ'' coding region after pTet in order to control the gene expression by adding inducer.This part does not pose any biological threat. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
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Latest revision as of 17:08, 21 October 2009
constitutive promoter-rbs-tetR(LVA)
This part used to construct a device which can generate repressor of pTet regulator consitutively. TetR repression is inhibited by the addition of tetracycline or its analog, aTc. We inserted cheZ coding region after pTet in order to control the gene expression by adding inducer.This part does not pose any biological threat.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]