Difference between revisions of "Part:BBa K283030"
 
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<partinfo>BBa_K283030 short</partinfo>
 
<partinfo>BBa_K283030 short</partinfo>
  
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This is a device that enable the control of chez(histag) using IPTG. The pLacIq promoter enables high level of expression of the LacI repressor protein. The LacI protein represses the Plac promoter. IPTG binds with LacI repressor protein and remove the inhibiting effect of the LacI on the pLac promoter.  
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This is a device that enable the control of ''cheZ''(histag) expression using IPTG. The pLacIq promoter enables high level of expression of the LacI repressor protein. The LacI protein represses the Plac promoter. IPTG binds with LacI repressor protein and remove the inhibiting effect of the LacI on the pLac promoter. This part does not pose any biological threat.
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caution:we confirmed K283008 in K283030 doesn't have sequence of RBS and stop codon.-Tokyo_Metropolitan 2011
  
 
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Latest revision as of 02:35, 6 October 2011

rbs-cheZ_histag-terminators

This is a device that enable the control of cheZ(histag) expression using IPTG. The pLacIq promoter enables high level of expression of the LacI repressor protein. The LacI protein represses the Plac promoter. IPTG binds with LacI repressor protein and remove the inhibiting effect of the LacI on the pLac promoter. This part does not pose any biological threat.

caution:we confirmed K283008 in K283030 doesn't have sequence of RBS and stop codon.-Tokyo_Metropolitan 2011

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 664
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]