Difference between revisions of "Part:BBa K4600001:Design"
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===Source=== | ===Source=== | ||
| − | The pump sequence used here, originates from the genomic sequence of E.coli K12 | + | The pump sequence used here, originates from the genomic sequence of E.coli K12, and was mapped in 1993. |
===References=== | ===References=== | ||
| + | Ma D, Cook DN, Alberti M, Pon NG, Nikaido H, Hearst JE. Molecular cloning and characterization of acrA and acrE genes of Escherichia coli. J Bacteriol. 1993 Oct;175(19):6299-313. doi: 10.1128/jb.175.19.6299-6313.1993. PMID: 8407802; PMCID: PMC206727. | ||
Latest revision as of 16:14, 26 September 2023
AcrB is a part of a multidrug efflux pump system found commonly in E.coli.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2830
Illegal PstI site found at 937 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2830
Illegal PstI site found at 937 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2830
Illegal XhoI site found at 773 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2830
Illegal PstI site found at 937 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2830
Illegal PstI site found at 937
Illegal NgoMIV site found at 859
Illegal NgoMIV site found at 1831
Illegal NgoMIV site found at 1864 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was already codon optimised.
Source
The pump sequence used here, originates from the genomic sequence of E.coli K12, and was mapped in 1993.
References
Ma D, Cook DN, Alberti M, Pon NG, Nikaido H, Hearst JE. Molecular cloning and characterization of acrA and acrE genes of Escherichia coli. J Bacteriol. 1993 Oct;175(19):6299-313. doi: 10.1128/jb.175.19.6299-6313.1993. PMID: 8407802; PMCID: PMC206727.