Difference between revisions of "Part:BBa K4806201:Design"
| Line 29: | Line 29: | ||
<p>The <sup>*</sup> marked parts were not created by us. Our results can be found on the experience page of each part.</p> | <p>The <sup>*</sup> marked parts were not created by us. Our results can be found on the experience page of each part.</p> | ||
</html> | </html> | ||
| − | |||
===References=== | ===References=== | ||
Latest revision as of 13:22, 8 October 2023
CYP3A4 gene with FLAG-tag for Chlamydomonas reinhardtii (Phytobrick)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1846
Illegal PstI site found at 2168
Illegal PstI site found at 2228
Illegal PstI site found at 2700
Illegal PstI site found at 2769
Illegal PstI site found at 2873 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 249
Illegal PstI site found at 1846
Illegal PstI site found at 2168
Illegal PstI site found at 2228
Illegal PstI site found at 2700
Illegal PstI site found at 2769
Illegal PstI site found at 2873 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 530
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1846
Illegal PstI site found at 2168
Illegal PstI site found at 2228
Illegal PstI site found at 2700
Illegal PstI site found at 2769
Illegal PstI site found at 2873 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1846
Illegal PstI site found at 2168
Illegal PstI site found at 2228
Illegal PstI site found at 2700
Illegal PstI site found at 2769
Illegal PstI site found at 2873
Illegal NgoMIV site found at 2090
Illegal NgoMIV site found at 3165
Illegal NgoMIV site found at 3171
Illegal NgoMIV site found at 3583
Illegal AgeI site found at 268 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo parts.
Source
This composite part is a combination of the following parts:
- 1. AβSAP(i)-promotor (A1-B2) (BBa_K4806013)
- 2. CYP3A4 gene (B3-B4) (BBa_K4806000)
- 3. FLAG-tag (B5) (BBa_K4806012)
- 4. tRPL23-terminator (B6-C1) (BBa_K3002006)*
- 1. Connector B2-B3 (BBa_K3002303)*
- 2. Connector B4-B5 (BBa_K3002304)*
- 3. Connector B5-B6 (BBa_K3002305)*
The * marked parts were not created by us. Our results can be found on the experience page of each part.