Difference between revisions of "Part:BBa K4806201:Design"

 
 
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===Design Notes===
 
===Design Notes===
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This part has been codon optimized for <i>Chlamydomonas reinhardtii</i> and it is compatible with Phytobricks/MoClo parts.
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===Source===
 
===Source===
  
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<html>
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<p>
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This composite part is a combination of the following parts:
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<ul>
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<li>1. AβSAP(i)-promotor (A1-B2) (<a href=" https://parts.igem.org/Part:BBa_K4806013">BBa_K4806013</a>)</li>
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<li>2. CYP3A4 gene (B3-B4) (<a href=" https://parts.igem.org/Part:BBa_K4806000">BBa_K4806000</a>)</li>
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<li>3. FLAG-tag (B5)  (<a href=" https://parts.igem.org/Part:BBa_K4806012">BBa_K4806012</a>)</li>
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<li>4. tRPL23-terminator (B6-C1) (<a href=" https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>)<sup>*</sup></li>
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</ul>
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Additionally, connectors were used to create the MoClo overhangs:
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<ul>
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<li>1. Connector B2-B3 (<a href=" https://parts.igem.org/Part:BBa_K3002303">BBa_K3002303</a>)<sup>*</sup></li>
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<li>2. Connector B4-B5 (<a href=" https://parts.igem.org/Part:BBa_K3002304">BBa_K3002304</a>)<sup>*</sup></li>
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<li>3. Connector B5-B6 (<a href=" https://parts.igem.org/Part:BBa_K3002305">BBa_K3002305</a>)<sup>*</sup></li>
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</ul>
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</p>
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<p>The <sup>*</sup> marked parts were not created by us. Our results can be found on the experience page of each part.</p>
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</html>
  
 
===References===
 
===References===

Latest revision as of 13:22, 8 October 2023


CYP3A4 gene with FLAG-tag for Chlamydomonas reinhardtii (Phytobrick)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1846
    Illegal PstI site found at 2168
    Illegal PstI site found at 2228
    Illegal PstI site found at 2700
    Illegal PstI site found at 2769
    Illegal PstI site found at 2873
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1846
    Illegal PstI site found at 2168
    Illegal PstI site found at 2228
    Illegal PstI site found at 2700
    Illegal PstI site found at 2769
    Illegal PstI site found at 2873
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 530
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1846
    Illegal PstI site found at 2168
    Illegal PstI site found at 2228
    Illegal PstI site found at 2700
    Illegal PstI site found at 2769
    Illegal PstI site found at 2873
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1846
    Illegal PstI site found at 2168
    Illegal PstI site found at 2228
    Illegal PstI site found at 2700
    Illegal PstI site found at 2769
    Illegal PstI site found at 2873
    Illegal NgoMIV site found at 2090
    Illegal NgoMIV site found at 3165
    Illegal NgoMIV site found at 3171
    Illegal NgoMIV site found at 3583
    Illegal AgeI site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo parts.

Source

This composite part is a combination of the following parts:

Additionally, connectors were used to create the MoClo overhangs:

The * marked parts were not created by us. Our results can be found on the experience page of each part.

References