Difference between revisions of "Part:BBa K4605007"
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To explore whether more colored fibers could be expressed in K.xylinus. We wanted to express EGFP in K.xylinus using a strong promoter (J23100) as a way to get fluorescent cellulose membranes.
 
To explore whether more colored fibers could be expressed in K.xylinus. We wanted to express EGFP in K.xylinus using a strong promoter (J23100) as a way to get fluorescent cellulose membranes.
 
==Experiments==
 
==Experiments==
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We chose to use the Anderson family promoter, first, because it is compatible with K.xylinus, and there is enough references to report that these promoters have been tested in several of bacteria such as G. xylinus 700178, G. hansenii 53582, and K. rhaeticus iGEM.Then we selected three of the strong promoters:J23100,J23104 and J23119.We linked it to bpsA in the hope that fluorescent cellulose could be expressed.
 
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 06:30, 8 October 2023


Strong promoters to improve the bpsA expression

Description

To explore whether more colored fibers could be expressed in K.xylinus. We wanted to express EGFP in K.xylinus using a strong promoter (J23100) as a way to get fluorescent cellulose membranes.

Experiments

We chose to use the Anderson family promoter, first, because it is compatible with K.xylinus, and there is enough references to report that these promoters have been tested in several of bacteria such as G. xylinus 700178, G. hansenii 53582, and K. rhaeticus iGEM.Then we selected three of the strong promoters:J23100,J23104 and J23119.We linked it to bpsA in the hope that fluorescent cellulose could be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]