Difference between revisions of "Part:BBa K4743003:Design"

 
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===References===
 
===References===
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1.Seifert, J., Kunz, N., Flachmann, R., Läufer, A., Jany, K. D., & Gassen, H. G. (1990). Expression of the E. coli nadB gene and characterization of the gene product L-aspartate oxidase. Biological chemistry Hoppe-Seyler, 371(3), 239–248.
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2.Cookson, B. T., Olivera, B. M., & Roth, J. R. (1987). Genetic characterization and regulation of the nadB locus of Salmonella typhimurium. Journal of bacteriology, 169(9), 4285–4293. https://doi.org/10.1128/jb.169.9.4285-4293.1987

Latest revision as of 08:59, 10 October 2023


NadB+ADH1 terminator with adapter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1833
    Illegal XbaI site found at 702
    Illegal XbaI site found at 921
    Illegal XbaI site found at 1167
    Illegal XbaI site found at 1264
    Illegal SpeI site found at 1638
    Illegal PstI site found at 1034
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1833
    Illegal SpeI site found at 1638
    Illegal PstI site found at 1034
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1833
    Illegal BamHI site found at 16
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1833
    Illegal XbaI site found at 702
    Illegal XbaI site found at 921
    Illegal XbaI site found at 1167
    Illegal XbaI site found at 1264
    Illegal SpeI site found at 1638
    Illegal PstI site found at 1034
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1833
    Illegal XbaI site found at 702
    Illegal XbaI site found at 921
    Illegal XbaI site found at 1167
    Illegal XbaI site found at 1264
    Illegal SpeI site found at 1638
    Illegal PstI site found at 1034
    Illegal AgeI site found at 1846
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 801


Design Notes

No specific consider for the design


Source

Listeria innocua (serotype 6a)

References

1.Seifert, J., Kunz, N., Flachmann, R., Läufer, A., Jany, K. D., & Gassen, H. G. (1990). Expression of the E. coli nadB gene and characterization of the gene product L-aspartate oxidase. Biological chemistry Hoppe-Seyler, 371(3), 239–248.

2.Cookson, B. T., Olivera, B. M., & Roth, J. R. (1987). Genetic characterization and regulation of the nadB locus of Salmonella typhimurium. Journal of bacteriology, 169(9), 4285–4293. https://doi.org/10.1128/jb.169.9.4285-4293.1987