Difference between revisions of "Part:BBa K4606011:Design"

 
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===Source===
 
===Source===
 
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Plasmids were obtained from Escherichia coli.
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===References===
 
===References===

Latest revision as of 02:17, 23 September 2023


Vector- Luciferase-SMAD4(WT)-AmpR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to the addition of the AmpR gene to the plasmid, we could easily confirm whether the recombination was successful or not, as only the recombinants can grow into colonies on the medium supplemented with Ampicillin.


Source

Plasmids were obtained from Escherichia coli.

References