Difference between revisions of "Part:BBa K4606004:Design"
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<partinfo>BBa_K4606004 short</partinfo>
 
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<partinfo>BBa_K4606004 SequenceAndFeatures</partinfo>
 
 
For detailed design information, you can look up the registry of (BBa_K 4606003), the device for gene segment of SMAD4.
 
For detailed design information, you can look up the registry of (BBa_K 4606003), the device for gene segment of SMAD4.
 
According to the base sequence information of SMAD4 in the database, we mutated the SMAD4 gene in a region that complementary binds to miR-3074-5p. By inserting its 3’UTR region (414bp) behind the luciferase gene in the vector, we could determine the expression level of this gene segment.
 
According to the base sequence information of SMAD4 in the database, we mutated the SMAD4 gene in a region that complementary binds to miR-3074-5p. By inserting its 3’UTR region (414bp) behind the luciferase gene in the vector, we could determine the expression level of this gene segment.
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<partinfo>BBa_K4606004 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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Based on the online repository of miRNA targets, SMAD4 might be a target of miR-3074-5p in osteoarthritis. Here we mutated the SMAD4 gene (wild type) in a region that complementary binds to miR-3074-5p.
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===Source===
 
===Source===
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The base sequences were derived from the database of Targetscan website.
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===References===
 
===References===

Latest revision as of 02:05, 23 September 2023


SMAD4(mutant type)

For detailed design information, you can look up the registry of (BBa_K 4606003), the device for gene segment of SMAD4. According to the base sequence information of SMAD4 in the database, we mutated the SMAD4 gene in a region that complementary binds to miR-3074-5p. By inserting its 3’UTR region (414bp) behind the luciferase gene in the vector, we could determine the expression level of this gene segment.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Based on the online repository of miRNA targets, SMAD4 might be a target of miR-3074-5p in osteoarthritis. Here we mutated the SMAD4 gene (wild type) in a region that complementary binds to miR-3074-5p.

Source

The base sequences were derived from the database of Targetscan website.

References