Difference between revisions of "Part:BBa K4606002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Firefly-luciferase is an ideal reporter method for gene detection. By inserting it into the tail of the target gene, the expression level of the target gene can be reflected according to the luciferase activity. | |
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===Source=== | ===Source=== | ||
| − | + | The firefly-luciferase gene sequense is from the Photinus pyralis genome. | |
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===References=== | ===References=== | ||
| + | Chen IY, Gheysens O, Ray S, et al. Indirect imaging of cardiac-specific transgene expression using a bidirectional two-step transcriptional amplification strategy. Gene Ther. 2010;17(7):827-838. doi:10.1038/gt.2010.30 | ||
Latest revision as of 01:58, 23 September 2023
Firefly-luciferase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Firefly-luciferase is an ideal reporter method for gene detection. By inserting it into the tail of the target gene, the expression level of the target gene can be reflected according to the luciferase activity.
Source
The firefly-luciferase gene sequense is from the Photinus pyralis genome.
References
Chen IY, Gheysens O, Ray S, et al. Indirect imaging of cardiac-specific transgene expression using a bidirectional two-step transcriptional amplification strategy. Gene Ther. 2010;17(7):827-838. doi:10.1038/gt.2010.30