Difference between revisions of "Part:BBa K4656001"
 
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<partinfo>BBa_K4656001 short</partinfo>
 
<partinfo>BBa_K4656001 short</partinfo>
  
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Tdc is the gene encoding tryptophan decarboxylase (TDC), which can remove the carboxyl group from 5-hydroxytryptophan (5-HTP) and convert it into serotonin (5-hydroxytryptophan, 5-HT) molecules. Tryptophan decarboxylase exists widely in animals and plants, especially in the central nervous system and gastrointestinal tract. Among them, 5-HTP is a precursor substance of neurotransmitter, while serotonin is an important neurotransmitter, similar to intestinal motor in terms of intestinal physiological function, and plays an important role in alleviating chronic constipation.  
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TDC can remove the carboxyl group from 5-hydroxytryptophan (5-HTP) and convert it into serotonin (5-hydroxytryptophan, 5-HT). Tryptophan decarboxylase exists widely in animals and plants, especially in the central nervous system and gastrointestinal tract. 5-HTP is a precursor substance of the neurotransmitter 5-HT, an important neurotransmitter that plays an intestinal motility role in the physiological function of the intestine and plays a vital role in alleviating chronic constipation.
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<html><BR><BR><center><img style="display: block;-webkit-user-select: none;margin: auto;cursor: zoom-in;background-color: hsl(0,0%,90%);transition: background-color 300ms;" src="https://static.igem.wiki/teams/4656/wiki/tdc-tph.jpg" width="600" height="200"></center></html>
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<center><b>The synthesis of 5-HT </b></center>
  
 
===Usage and Biology===
 
===Usage and Biology===
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Our project uses the introduction of Tdc sequences and inducing their expression to produce serotonin to test their efficacy in treating constipation. The original sequence of TDC was derived from the genome of the organism Oryza sativa Japonica Group (Japanese rice).
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In our 5-HT producing circuit, TDC and TPH together catalyze the generation of 5-HT, when sensing a decrease in butyrate concentration. Experimental results about our 5-HT producing circuit can be found in our composite part BBa K4656007.
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<html><BR><BR><center><img style="display: block;-webkit-user-select: none;margin: auto;cursor: zoom-in;background-color: hsl(0,0%,90%);transition: background-color 300ms;" src="https://static.igem.wiki/teams/4656/wiki/part-plam-tph-tdc.jpg" width="600" height="300"></center></html>
  
 
===Sequence and Features===
 
===Sequence and Features===

Latest revision as of 12:28, 5 October 2023


TDC

TDC can remove the carboxyl group from 5-hydroxytryptophan (5-HTP) and convert it into serotonin (5-hydroxytryptophan, 5-HT). Tryptophan decarboxylase exists widely in animals and plants, especially in the central nervous system and gastrointestinal tract. 5-HTP is a precursor substance of the neurotransmitter 5-HT, an important neurotransmitter that plays an intestinal motility role in the physiological function of the intestine and plays a vital role in alleviating chronic constipation.

The synthesis of 5-HT

Usage and Biology

In our 5-HT producing circuit, TDC and TPH together catalyze the generation of 5-HT, when sensing a decrease in butyrate concentration. Experimental results about our 5-HT producing circuit can be found in our composite part BBa K4656007.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1248
    Illegal BamHI site found at 732
    Illegal BamHI site found at 834
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 340
    Illegal NgoMIV site found at 775
    Illegal NgoMIV site found at 1330
    Illegal AgeI site found at 478
    Illegal AgeI site found at 1402
  • 1000
    COMPATIBLE WITH RFC[1000]