Difference between revisions of "Part:BBa K4656000"

Latest revision as of 11:41, 5 October 2023

PchA

The pchA is present in the butyrate sensor derived from the EHEC strain O157: H7, which was discovered by Nakanishi et al. (2009)[1]. The butyrate sensing system includes sequences of pchA promoters (PpchA) and pchA regulatory factor located upstream, and LEE1 promoters (PLee1) and RBS located downstream. Nakanishi et al. finds that leucine response regulatory protein (Lrp) forms a complex with butyrate, which activates transcription of the pchA regulatory factor after binding to the PpchA. The pchA regulatory factor then binds to the PLee1 to transcribe the ler gene. In short, it is a regulatory gene(regulates LEE1 gene, encodes pchA protein).

Usage and Biology

In our butyrate-sensing circuit, pchA is controlled by PpchA，which can sense changes in butyrate concentration. PchA binds the promoter of LEE1, PLEE1, and then helps to activate the expression of its downstream gene. Experimental results about our butyrate-sensing circuit can be found in our composite part BBa K4656008.

Reference

[1]. Nakanishi, N., et al., Regulation of virulence by butyrate sensing in enterohaemorrhagic Escherichia coli. Microbiology (Reading), 2009. 155(Pt 2): p. 521-530.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 268
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4656000 short</partinfo>
-The pchA is present in the butyrate sensor derived from the EHEC strain O157: H7, which was discovered by Nakanishi et al. (2009)[1]. The butyrate sensing system includes sequences of pchA promoters&#65288;PpchA&#65289; and pchA regulators located upstream, and LEE1 promoters&#65288;PLee1&#65289; and RBS located downstream. Nakanishi et al. found that leucine response regulatory protein (Lrp) forms a complex with butyrate, which activates the transcription of pchA regulatory factors after binding to the PpchA. The pchA regulator then binds to the PLee1 to transcribe the ler gene.
+The pchA is present in the butyrate sensor derived from the EHEC strain O157: H7, which was discovered by Nakanishi et al. (2009)[1]. The butyrate sensing system includes sequences of pchA promoters (PpchA) and pchA regulatory factor located upstream, and LEE1 promoters (PLee1) and RBS located downstream. Nakanishi et al. finds that leucine response regulatory protein (Lrp) forms a complex with butyrate, which activates transcription of the pchA regulatory factor after binding to the PpchA. The pchA regulatory factor then binds to the PLee1 to transcribe the ler gene.
 In short, it is a regulatory gene(regulates LEE1 gene, encodes pchA protein).
-In our genetic circuit, pchA is used to bind the promoter of LEE1, PLEE1, and then help to activate the expression of its downstream CI gene.
 ===Usage and Biology===
-In our genetic circuit, pchA is used to bind the promoter of LEE1, PLEE1, and then help to activate the expression of its downstream gene.
+In our butyrate-sensing circuit, pchA is controlled by PpchA，which can sense changes in butyrate concentration. PchA binds the promoter of LEE1, PLEE1, and then helps to activate the expression of its downstream gene. Experimental results about our butyrate-sensing circuit can be found in our composite part BBa K4656008.
+<html><BR><BR><center><img style="display: block;-webkit-user-select: none;margin: auto;cursor: zoom-in;background-color: hsl(0,0%,90%);transition: background-color 300ms;" src="https://static.igem.wiki/teams/4656/wiki/part-pcha-plee1-ci.jpg" width="600" height="300"></center></html>
 ===Reference===
-[1]. Nakanishi, N., et al., Regulation of virulence by butyrate sensing in enterohaemorrhagic Escherichia  coli. Microbiology (Reading), 2009. 155(Pt 2): p. 521-530.
+[1]. Nakanishi, N., et al., Regulation of virulence by butyrate sensing in enterohaemorrhagic Escherichia coli. Microbiology (Reading), 2009. 155(Pt 2): p. 521-530.
 ===Sequence and Features===