Difference between revisions of "Part:BBa K245112:Design"
(→Source) |
|||
(One intermediate revision by the same user not shown) | |||
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K245112 short</partinfo> | <partinfo>BBa_K245112 short</partinfo> | ||
Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
− | + | The part is inserted in BB-NIC-II-HisN vector (BBa_K245005) and can be assembled with other parts according to assembly standard 37. Composite parts were assembled (iGEM 2009, team Slovenia) by cutting front inserts with EcoRI and BspEI and front vectors with EcoRI and NgoMIV, thus forming RFC 37 Scar Sequence (BBa_K245009) beween separate parts. | |
− | + | ||
− | + | ||
===Source=== | ===Source=== | ||
− | + | Composed from P5 basic part and P2-H1 intermediate part according to assembly standard 37. | |
===References=== | ===References=== |
Latest revision as of 20:02, 21 October 2009
P5-P2-H1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part is inserted in BB-NIC-II-HisN vector (BBa_K245005) and can be assembled with other parts according to assembly standard 37. Composite parts were assembled (iGEM 2009, team Slovenia) by cutting front inserts with EcoRI and BspEI and front vectors with EcoRI and NgoMIV, thus forming RFC 37 Scar Sequence (BBa_K245009) beween separate parts.
Source
Composed from P5 basic part and P2-H1 intermediate part according to assembly standard 37.