Difference between revisions of "Part:BBa K4676001:Design"
(→Design Notes) |
|||
| (One intermediate revision by the same user not shown) | |||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | The sequence for this part was kept the same as found naturally in the sequence of Glyphotaelius pellucidus. | + | The sequence for this part was kept the same as found naturally in the sequence of Glyphotaelius pellucidus, except for his-tag and sumo sequences being added to the c-terminus of the l-fibroin, in order to allow for protein purification. Furthermore, this sequence has been codon optimized using the biotechnology platform Benchling. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 21:49, 11 October 2023
L-Fibroin of G. Pellucidus
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence for this part was kept the same as found naturally in the sequence of Glyphotaelius pellucidus, except for his-tag and sumo sequences being added to the c-terminus of the l-fibroin, in order to allow for protein purification. Furthermore, this sequence has been codon optimized using the biotechnology platform Benchling.
Source
The sequence was taken from the genome of Glyphotaelius pellucidus, which is uploaded to the NCBI BLAST database.