Difference between revisions of "Part:BBa K4887001:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | In order to construct the IbGBSSI knockout system, the sequence of gene IbGBSSI must be understood clearly so that the appropriate sgRNA (BBa_K4887002) could be designed targeting for the gene IbGBSSI. | |
− | + | ||
===Source=== | ===Source=== | ||
− | + | AB071604(Accession umber of NCBI GenBank), from sweet potato (Ipomoea batatas) genomic sequence. | |
+ | |||
===References=== | ===References=== |
Latest revision as of 06:26, 5 October 2023
IbGBSSI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1459
Illegal BsaI.rc site found at 123
Illegal SapI site found at 1156
Illegal SapI.rc site found at 1225
Design Notes
In order to construct the IbGBSSI knockout system, the sequence of gene IbGBSSI must be understood clearly so that the appropriate sgRNA (BBa_K4887002) could be designed targeting for the gene IbGBSSI.
Source
AB071604(Accession umber of NCBI GenBank), from sweet potato (Ipomoea batatas) genomic sequence.