Difference between revisions of "Part:BBa K4890001:Design"

 
 
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===Design Notes===
 
===Design Notes===
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We aim to construct Drosophila lines to detect heavy metals in the environment. MTF-1 is used to trigger the response of Drosophila to heavy metal ions. MTF-1 is a transcription factor that can be activated by heavy metals. In the cell nucleus of Drosophila cells, MTF-1 binds to MRE to recruit RNA polymerase, in turn increasing the expression of Mto/Mtn to detoxicate metals. We replaced Mto/Mtn with Hid (head involution defective) to visualize transcription process.  
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===Source===
 
===Source===
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The source of the part will be its sequence which was retrieved from flybase.org.
  
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===References===
 
===References===

Latest revision as of 10:20, 5 October 2023


MTF1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 1923
    Illegal PstI site found at 1681
    Illegal PstI site found at 1859
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 1923
    Illegal NheI site found at 136
    Illegal PstI site found at 1681
    Illegal PstI site found at 1859
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 1923
    Illegal BglII site found at 154
    Illegal BamHI site found at 106
    Illegal BamHI site found at 1095
    Illegal BamHI site found at 1491
    Illegal BamHI site found at 1518
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 1923
    Illegal PstI site found at 1681
    Illegal PstI site found at 1859
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 1923
    Illegal PstI site found at 1681
    Illegal PstI site found at 1859
    Illegal NgoMIV site found at 1357
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We aim to construct Drosophila lines to detect heavy metals in the environment. MTF-1 is used to trigger the response of Drosophila to heavy metal ions. MTF-1 is a transcription factor that can be activated by heavy metals. In the cell nucleus of Drosophila cells, MTF-1 binds to MRE to recruit RNA polymerase, in turn increasing the expression of Mto/Mtn to detoxicate metals. We replaced Mto/Mtn with Hid (head involution defective) to visualize transcription process.


Source

The source of the part will be its sequence which was retrieved from flybase.org.


References