Difference between revisions of "Part:BBa K4790044"
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<partinfo>BBa_K4790044 short</partinfo> | <partinfo>BBa_K4790044 short</partinfo> | ||
| − | The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on | + | The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on node 59 and coding the 46th amino acid from tyrosine to isoleucine. According to Codon preference in E.coli, ATT has the highest possibility coding isoleucine. |
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Latest revision as of 12:11, 4 October 2023
ASR1-Y46I-F
The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on node 59 and coding the 46th amino acid from tyrosine to isoleucine. According to Codon preference in E.coli, ATT has the highest possibility coding isoleucine.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]