Difference between revisions of "Part:BBa K4765021"
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| − | ==Introduction== | + | <html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html> |
| + | __TOC__ | ||
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| + | ===Introduction=== | ||
The stem-loop is a piece of RNA that forms the stem loop structure, which is located at the 3' end of the mono-cistron cut by ribozyme, preventing the degradation of mono-cistron mRNA.<ref> Liu, Y., Wu, Z., Wu, D., Gao, N., & Lin, J. (2022). Reconstitution of Multi-Protein Complexes through Ribozyme-Assisted Polycistronic Co-Expression. ''ACS Synthetic Biology, 12''(1), 136–143. https://doi.org/10.1021/acssynbio.2c00416</ref>. | The stem-loop is a piece of RNA that forms the stem loop structure, which is located at the 3' end of the mono-cistron cut by ribozyme, preventing the degradation of mono-cistron mRNA.<ref> Liu, Y., Wu, Z., Wu, D., Gao, N., & Lin, J. (2022). Reconstitution of Multi-Protein Complexes through Ribozyme-Assisted Polycistronic Co-Expression. ''ACS Synthetic Biology, 12''(1), 136–143. https://doi.org/10.1021/acssynbio.2c00416</ref>. | ||
| − | ==Usage and Biology== | + | ===Usage and Biology=== |
| − | + | In the 2022 iGEM competition, we successfully constructed pRAP using the hammerhead ribozyme to modify the metabolism of ''E. coli'' for β-carotene production. This year, we have discovered that the mono-cistron mRNA generated by the ribozyme self-cleavage is unstable. Therefore, we have added stem-loop 1 at the 3' end of the corresponding CDS to prevent the degradation of mono-cistron mRNA. | |
| − | + | ||
| + | ===Characterization=== | ||
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<partinfo>BBa_K4765021 parameters</partinfo> | <partinfo>BBa_K4765021 parameters</partinfo> | ||
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==References== | ==References== | ||
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Latest revision as of 12:23, 12 October 2023
stem-loop 1, from 10.1021/acssynbio.2c00416
Introduction
The stem-loop is a piece of RNA that forms the stem loop structure, which is located at the 3' end of the mono-cistron cut by ribozyme, preventing the degradation of mono-cistron mRNA.[1].
Usage and Biology
In the 2022 iGEM competition, we successfully constructed pRAP using the hammerhead ribozyme to modify the metabolism of E. coli for β-carotene production. This year, we have discovered that the mono-cistron mRNA generated by the ribozyme self-cleavage is unstable. Therefore, we have added stem-loop 1 at the 3' end of the corresponding CDS to prevent the degradation of mono-cistron mRNA.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Liu, Y., Wu, Z., Wu, D., Gao, N., & Lin, J. (2022). Reconstitution of Multi-Protein Complexes through Ribozyme-Assisted Polycistronic Co-Expression. ACS Synthetic Biology, 12(1), 136–143. https://doi.org/10.1021/acssynbio.2c00416