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The stem-loop is a piece of RNA that forms the stem loop structure, which is located at the 3' end of the mono-cistron cut by ribozyme, preventing the degradation of mono-cistron mRNA<ref> Liu, Y., Wu, Z., Wu, D., Gao, N., & Lin, J. (2022). Reconstitution  of Multi-Protein Complexes through Ribozyme-Assisted Polycistronic  Co-Expression. ’‘ACS Synthetic Biology, 12’‘(1), 136–143. https://doi.org/10.1021/acssynbio.2c00416</ref>.
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html>
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===Introduction===
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The stem-loop is a piece of RNA that forms the stem loop structure, which is located at the 3' end of the mono-cistron cut by ribozyme, preventing the degradation of mono-cistron mRNA.<ref> Liu, Y., Wu, Z., Wu, D., Gao, N., & Lin, J. (2022). Reconstitution  of Multi-Protein Complexes through Ribozyme-Assisted Polycistronic  Co-Expression. ''ACS Synthetic Biology, 12''(1), 136–143. https://doi.org/10.1021/acssynbio.2c00416</ref>.
  
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===Usage and Biology===
 
===Usage and Biology===
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In the 2022 iGEM competition, we successfully constructed pRAP using the hammerhead ribozyme to modify the metabolism of ''E. coli'' for β-carotene production. This year, we have discovered that the mono-cistron mRNA generated by the ribozyme self-cleavage is unstable. Therefore, we have added stem-loop 1 at the 3' end of the corresponding CDS to prevent the degradation of mono-cistron mRNA.
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===Characterization===
  
 
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<span class='h3bb'>Sequence and Features</span>
 
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===Functional Parameters===
 
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==References==
 
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Latest revision as of 12:23, 12 October 2023


stem-loop 1, from 10.1021/acssynbio.2c00416

contributed by Fudan iGEM 2023

Introduction

The stem-loop is a piece of RNA that forms the stem loop structure, which is located at the 3' end of the mono-cistron cut by ribozyme, preventing the degradation of mono-cistron mRNA.[1].

Usage and Biology

In the 2022 iGEM competition, we successfully constructed pRAP using the hammerhead ribozyme to modify the metabolism of E. coli for β-carotene production. This year, we have discovered that the mono-cistron mRNA generated by the ribozyme self-cleavage is unstable. Therefore, we have added stem-loop 1 at the 3' end of the corresponding CDS to prevent the degradation of mono-cistron mRNA.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. ↑ Liu, Y., Wu, Z., Wu, D., Gao, N., & Lin, J. (2022). Reconstitution of Multi-Protein Complexes through Ribozyme-Assisted Polycistronic Co-Expression. ACS Synthetic Biology, 12(1), 136–143. https://doi.org/10.1021/acssynbio.2c00416